GRB2 抗体 (C-Term)
Quick Overview for GRB2 抗体 (C-Term) (ABIN374150)
抗原
See all GRB2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-PRNYVTPVNR NV
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特异性
- This antibody detects GRB2 / ASH at C-term.
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交叉反应 （详细）
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Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse -
纯化方法
- Immunoaffinity Chromatography
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免疫原
- Synthetic peptide from C-Terminus of the protein sequence according to NP_002077.1, NP_987102.1 Genename: GRB2
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anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibody
GRB2 适用: 人, 小鼠, 大鼠, 犬, 小鸡 ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibodyVerified GRB2 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibodyKO Validated GRB2 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 89-118) antibodyGRB2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 156-217) antibodyGRB2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 适用: 人, 小鼠, 大鼠, Cow, Hamster WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (N-Term) antibodyGRB2 适用: 人, 小鼠, 大鼠, 犬, Cow, 斑马鱼, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (AA 1-217) antibodyGRB2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Growth Factor Receptor-Bound Protein 2 (GRB2) antibodyGRB2 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 6E1 unconjugated Recombinant Antibody
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in Jurkat lysate. Immunohistochemistry: 0.1-0.3 μg/mL. In paraffin embedded Human Tonsil, stains mantlezone and to a lessser extent germinal centre.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))
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别名
- GRB2 / ASH
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背景
- Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2
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基因ID
- 2885
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NCBI登录号
- NP_002077
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UniProt
- P62993
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途径
- RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation
抗原
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